- Cytometry and Antibody Technology Facility
Department of Medicine
- Fitch Monoclonal Subcore
- Flow Cytometry Subcore
The CAT facility serves the UChicago Faculty by providing all of the tools necessary to measure the properties of cells and their products. The Fitch Monoclonal Subcore (FMS) develops the reagents used to label the cells (novel monoclonal antibody production, purification of existing monoclonals, and fluorescently-coupled antibodies), whereas the Flow Cytometry Subcore (FCS) provides the technology and staff expertise to use these reagents for experimental measurements (flow cytometers, image cytometers, and cell sorters). Antibody technology and its usage have evolved concurrently with flow cytometry and has become the dominant tool for the identification and classification of cells and cellular products. The CAT Facility provides instrumentation and antibodies for the analytical detection of subcellular components and proteins using a range of fluorescence and non-fluorescence-based detection technologies. Further, the CAT Facility is designed to meet the wide-spread needs for specialized cytometric analysis and continues to respond to the demand for new and improved technology. Among these improvements include an Antibody/Hybridoma Bank that provides commonly-used, highest-quality purified antibodies, pre-titered conjugated antibodies for flow cytometry, and hybridoma cell lines.
The Facility's mission is three-fold: We aim to provide for our users:
(1) cutting-edge technology operated by expertly trained personnel;
(2) high-quality analytical monoclonal antibodies; and
(3) expert technical consultation and training of researchers.